The present study aims to investigate the sialic acid content in colostrum of two crossbred goats, Sannen ×× Beetal and Alpine × Beetal, and to compare the effect of milking time on the sialic acid content between the breeds. Colostrum samples were collected from all the animals, at an interval of 12 hours, beginning at kidding

till 72 hours thereafter. The sialic acid concentrations were estimated from the processed colostrum samples by fluorimetric method. The sialic acid content in colostrum of Alpine × Beetle and Sannen × Beetle goats ranged from 0.19 to 1.03 and 0.17 to 0.98 mg/ml, respectively. Sialic acid concentrations of colostrum in both the breeds were higher at 0 hour of milking and a gradual decrease in their levels was observed till 72 hours of milking. The mean ± S.E. sialic acid concentrations of Alpine × Beetal colostrum at 12, 24, 36, 48, 60 and 72 hours were significantly (P < 0.001) higher than their respective values for Sannen × Beetle colostrum. This study indicated that the breed and milking time have significant effect on sialic acid concentration of goat’s colostrum.